Formation of melanin-based wing patterns is influenced by condition and immune challenge in Pieris brassicae

According to life-history theory, trade-offs emerge because organisms possess a limited amount of resources that they have to allocate between different bodily functions. Here, we tested whether there is a trade-off between melanin-based immune response and dark melanized wing patterning in the large white butterfly, Pieris brassicae L. (Lepidoptera: Pieridae), by activating the immune system of pupae and measuring the wing pigmentation of freshly emerged adults. In contrast to expectations, we did not find any negative associations between immune challenge and wing patterning. Furthermore, implanted and punctured male pupae tended to have larger and darker forewing tips as adults compared to controls. Both in females and males, different wing spots were affected by conditionreflecting traits (e.g., pupal mass, brood), which suggest that formation of wing patterns may be a condition-dependent process and/or heritable.